The ability to engineer microvasculature would be of great utility for the tissue engineering of highly metabolic tissues such as myocardium. In addition, the alignment of such a network is critical to the success of using it to deliver oxygen to tissue cells because it provides natural inlet and outlet sides for perfusion. In this work, the ability of engineered microvessels to align with their fibrin gel matrix has been examined and a bioreactor has been designed to harness this ability and perfuse the engineered tissue. The results indicate that engineered microvasculature can be aligned via cell-induced gel compaction and that this compaction improves lumen density. Interstitial flow provided an additional increase in lumen density. A mathematical model of fluid flow through engineered microvessels and a variety of image analysis methods were also developed.
University of Minnesota Ph.D. dissertation. Ph.D. November 2012. Major:Biomedical Engineering. Advisor: Robert T. Tranquillo. 1 computer file (PDF); iv, 301 pages, appendices A-C.
Morin, Kristen Thatcher.
The development and alignment of engineered microvasculature in fibrin gel.
Retrieved from the University of Minnesota Digital Conservancy,
Content distributed via the University of Minnesota's Digital Conservancy may be subject to additional license and use restrictions applied by the depositor.