Cryopreservation is a process where cells or tissues are preserved by cooling to sub-zero temperatures, where any enzymatic or chemical reaction is stopped due to lack of thermal energy. Previous experiments studied the changes in cells at one specific layer. However, formation of ice on the surface, then into cell during the cooling process will affect image analysis. In this study, the physiochemical changes in Jurkat cells at three different layers were studied based on the distribution of ice, cryoprotectant, and cytochrome C using Raman Spectroscopy.
This research was supported by the Undergraduate Research Opportunities Program (UROP).
Yu, Guanglin; Yap, Yan Rou; Hubel, Allison.
Understanding physiochemical changes in Jurkat cells at different layers during freezing using Raman Spectroscopy.
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