Barley (Hordeum vulgare L.) is cultivated throughout the world, and the majority is utilized in the malting and brewing industry. The development of an agronomically competitive variety of low temperature tolerant (LTT) barley that could be sown in autumn and harvested in early July would be beneficial to farmers in Minnesota because it opens up the possibility of double cropping with peas and beans. Additionally, fall-sown barley provides certain advantages over spring cultivars, including increased yield and quality, as well as avoidance of high temperature, water stress, and diseases such as stem rust and Fusarium head blight (Muñoz-Amatriaín et al., 2009). To increase the diversity of LTT in cultivated barley, this study evaluated the survival rates of accessions from the Wild Barley Diversity Collection (WBDC) following a freeze chamber treatment and a field trial. Association mapping was utilized to identify and map loci conferring LTT.