Endosseous implants are important options for restoring edentulous dental sites. Patients
and practitioners are interested in implant systems that can osseointegrate faster and
predictably in compromised patients. Recent research has shown that utilizing
biomimetic peptides (i.e. RGD and SNa15) on titanium surfaces could improve
osteoblastic responses. RGD is a peptide sequence important in binding nearly half of all
integrins and is important in cellular adhesion. SNa15 is a modified peptide sequence
from the N-terminus of the salivary protein statherin, which has been shown to be
important in calcium phosphate mineral binding and nucleation. The aim of this study
was to compare the in vitro outcomes associated with osseointegration (osteoblast-like
cell adhesion, spreading, proliferation, and differentiation) between the titanium surfaces
coated with RGD, SNa15, or a combination of the two biomimetic peptides via a
covalently bound elastin-like biopolymer (p-). The co-immobilized titanium surfaces had
either a 1:1 molar ratio of biomimetic peptides on separate biopolymers (pRGD and
pSNa15) or a coating containing both peptides on a single biopolymer (pRGD-SNa15).
Cellular adhesion, spreading, and proliferation was assessed with fluorescent labeling of
actin, vinculin, and/or nuclear DNA and viewed with a light microscope. ELISA was
used to determine early- and late-stage osteoblastic differentiation by measuring alkaline
phosphatase and osteocalcin, respectively. Our results indicate that while titanium
surfaces coated with only pRGD displayed greater cell adhesion, there were nonsignificant
differences between titanium surfaces coated with elastin-like peptide
polymers in terms of other osseointegration-related outcomes. We also noted a pattern
that titanium surfaces coated with hydrophobic RGD-containing polymers were
associated with an abnormal late stage osteoblastic differentiation pattern. In conclusion,
we suggest that the co-immobilization of these biomimetic peptides via elastin-like
polymers should be optimized in further research to improve their clinical applicability.
University of Minnesota M.S. thesis. May 2012. Major: Dentistry. Advisor: Conrado Aparicio. 1 computer file (PDF); v, 84 pages.
Beckman, Eric Martin.
Osteoblast-like cell response to titanium coated with co-immobilized Elastin-like polymers containing integrin-binding and Statherin-derived biomimetic peptides.
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