Mesenchymal stromal cells (MSCs) are a population of mesoderm-derived cells that
possess the ability to differentiate into bone, cartilage, and adipose tissue. They are
important to understanding the developmental process of musculoskeletal tissue, and can
be utilized for novel human cell therapies. Previous studies by our group and others have
demonstrated development of MSCs from human embryonic stem cells (hESCs). Now,
we have identified a population of potential mesenchymal precursor cells from adult bone
marrow derived iPSC lines using CD73 as a selection marker. Sorting and culture of the
hESC/iPSC-derived CD73-positive cells lead to development of MSCs capable of
making bone, cartilage, and adipocytes. However the variations in differentiation
methods have been found to strongly influence their mesenchyme induction and their
ability to make bone, cartilage and adipose tissue. We compare the spin EB (embryoid
body) versus stromal co-culture techniques to arrive at a MSC population in our studies.
Additionally, these studies examined novel ways to derive iPSCs that could be used for
derivation of MSCs and other cell populations. Induced pluripotent stem cells have
previously been generated from human dermal fibroblast cells. However, the requirement
for skin biopsies and the need to expand fibroblast cells for several passages in vitro
make it a cumbersome source for generating patient-specific stem cells. Reprogramming
from human blood cells represents a consistent method of establishing patient-specific iPSCs.
University of Minnesota M.S. thesis. December 2011. Major: Stem cell biology. Advisor: Dan S. Kaufman. 1 computer file (PDF); iv, 32 pages.
Generation of induced pluripotent stem cells and mesenchymal stromal cells..
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